doi: 10.3969/j.issn.1001-3849.2025.10.018基于电聚合分子印迹聚合膜的卡那霉素电化学发光传感器-《电镀与精饰》

文章信息/Info

Title:: Electrochemiluminescence sensor for kanamycin based on electropolymerized molecularly imprinted polymer film

Author(s):: Li Xintong; Li Xinrui; Zhang Xinyu; Han Yongbin; Lian Wenjing*; (College of Basic Science, Tianjin Agricultural University, Tianjin 300392, China)

Keywords:: molecularly imprinted polymer; electrochemical polymerization; electrochemiluminescence; kanamycin

摘要:: 将分子印迹聚合物(MIP)的特异选择性与简便、灵敏的电化学发光(ECL)检测方法相结合构建卡那霉素(KA)分子印迹ECL传感器。通过电化学聚合方法在金电极表面以吡咯(PY)为功能单体，KA为模板分子合成MIP膜。以常用的发光试剂Ru(bpy)32+作为ECL探针，并加入共反应剂三丙胺(TPA)以提高传感器的检测灵敏度。通过MIP洗脱后与重结合KA的ECL响应差值对KA进行定量分析。该传感器对KA表现出高选择性、良好的重现性以及稳定性，其线性范围为5.00×10–8～1.00×10–5 mol·L–1，最低检出限为1.67×10–8 mol·L–1(S/N=3)，为开发简便且灵敏的分子印迹ECL传感器提供新思路。

Abstract:: In this work, by combining the specific selectivity of molecularly imprinted polymer (MIP) with a simple and sensitive electrochemiluminescence (ECL) detection method, a molecularly imprinted ECL sensor for kanamycin (KA) was developed. The MIP film was synthesized on the surface of gold electrode via electrochemical polymerization, using pyrrole (PY) as the functional monomer and KA as the template molecule. The commonly used luminescent reagent Ru(bpy)32+ was employed as the ECL probe, and its co-reagent tripropylamine (TPA) was added to enhance the detection sensitivity of the sensor. Quantitative analysis of KA was performed by evaluating the difference in ECL responses between MIP film electrodes after KA removal and KA rebinding. The sensor exhibited high selectivity, good reproducibility, and stability toward KA, with a linear range of 5.00×10? 8 to 1.00×10? 5 mol·L? 1 and a limit of detection of 1.67×10? 8 mol·L? 1 ( S/N=3). This work would provide a new idea for the development of simple and sensitive molecularly imprinted ECL sensors